(i) For any OSHA health standard, only the requirement to limit employee exposure to the specific permissible exposure limit shall apply for laboratories, unless that particular standard states otherwise or unless the conditions of paragraph (a)(2)(iii) of this section apply.
(ii) Prohibition of eye and skin contact where specified by any OSHA health standard shall be observed.
(iii) Where the action level (or in the absence of an action level, the permissible exposure limit) is routinely exceeded for an OSHA regulated substance with exposure monitoring and medical surveillance requirements paragraphs (d) and (g)(1)(ii) of this section shall apply.
(i) Uses of hazardous chemicals which do not meet the definition of laboratory use, and in such cases, the employer shall comply with the relevant standard in 29 CFR part 1910, subpart Z, even if such use occurs in a laboratory.
(ii) Laboratory uses of hazardous chemicals which provide no potential for employee exposure. Examples of such conditions might include:
- A) Procedures using chemically-impregnated test media such as Dip-and-Read tests where a reagent strip is dipped into the specimen to be tested and the results are interpreted by comparing the color reaction to a color chart supplied by the manufacturer of the test strip; and
- (B) Commercially prepared kits such as those used in performing pregnancy tests in which all of the reagents needed to conduct the test are contained in the kit.
"Action level" means a concentration designated in 29 CFR part 1910 for a specific substance, calculated as an eight (8)-hour time-weighted average, which initiates certain required activities such as exposure monitoring and medical surveillance.
"Assistant Secretary" means the Assistant Secretary of Labor for Occupational Safety and Health, U.S. Department of Labor, or designee.
"Carcinogen" (see "select carcinogen").
"Chemical Hygiene Officer" means an employee who is designated by the employer, and who is qualified by training or experience, to provide technical guidance in the development and implementation of the provisions of the Chemical Hygiene Plan. This definition is not intended to place limitations on the position description or job classification that the designated individual shall hold within the employer's organizational structure.
"Chemical Hygiene Plan" means a written program developed and implemented by the employer which sets forth procedures, equipment, personal protective equipment and work practices that (i) are capable of protecting employees from the health hazards presented by hazardous chemicals used in that particular workplace and (ii)meets the requirements of paragraph (e) of this section. "Combustible liquid" means any liquid having a flashpoint at or above100 ° F (37.8 ° C), but below 200 ° F (93.3 ° C),except any mixture having components with flashpoints of 200 °F (93.3 ° C), or higher, the total volume of which make up99 percent or more of the total volume of the mixture.
"Compressed gas" means: (i) A gas or mixture of gases having, in a container, an absolute pressure exceeding 40 psi at 70 ° F (21.1 ° C); or (ii) A gas or mixture of gases having, in a container, an absolute pressure exceeding 104 psi at 130 ° F (54.4 ° C) regardless of the pressure at 70 °F (21.1 ° C); or (iii) A liquid having a vapor pressure exceeding40 psi at 100 ° F (37.8 ° C) as determined by ASTM D-323-72.
"Designated area" means an area which may be used for work with "select carcinogens," reproductive toxins or substances which have a high degree of acute toxicity. A designated area may be the entire laboratory, such as a laboratory hood.
"Emergency" means any occurrence such as, but not limited to, equipment failure, rupture of containers or failure of control equipment which results in an uncontrolled release of a hazardous chemical into the workplace.
"Employee" means an individual employed in a laboratory workplace who may be exposed to hazardous chemicals in the course of his or her assignments.
"Explosive" means a chemical that causes a sudden, almost instantaneous release of pressure, gas, and heat when subjected to sudden shock, pressure, or high temperature.
"Flammable" means a chemical that falls into one of the following categories:
"Laboratory" means a facility where the "laboratory use of hazardous chemicals" occurs. It is a workplace wherer relatively small quantities of hazardous chemicals are used on a non-production basis.
"Laboratory scale" means work with substances in which he containers used for reactions, transfers, and other handling of substances are designed to be easily and safety manipulated by one person.
"Laboratory scale" excludes those workplaces whose function is to produce commercial quantities of materials. "Laboratory-typehood" means a device located in a laboratory, enclosure on five sides with a movable sash or fixed partial enclosed on the remaining side; constructed and maintained to draw air from the laboratory and to prevent or minimize the escape of air contaminants into the laboratory; and allows chemical manipulations to be conducted in the enclosure without insertion of any portion of the employee's body other than hands and arms. Walk-in hoods with adjustable sashes meet the above definition provided that the sashes are adjusted during use so that the airflow and the exhaust of air contaminants are not compromised and employees do not work inside the enclosure during the release of airborne hazardous chemicals.
"Laboratory use of hazardous chemicals" means handling or use of such chemicals in which all of the following conditions are met:
"Organic peroxide" means an organic compound that contains the bivalent -O-O- structure and which may be considered to be a structural derivative of hydrogen peroxide where one or both of the hydrogen atoms has been replaced by an organic radical.
"Oxidizer" means a chemical other than a blasting agent or explosive as defined in 1910.109(a), that initiates or promotes combustion in other materials, thereby causing fire either of itself or through the release of oxygen or other gases.
"Physical hazard" means a chemical for which there is scientifically valid evidence that it is a combustible liquid, a compressed gas, explosive, flammable, an organic peroxide, an oxidizer pyrophoric, unstable (reactive)or water-reactive.
"Protective laboratory practices and equipment" means those laboratory procedures, practices and equipment accepted by laboratory health and safety experts as effective, or that the employer can show to be effective, in minimizing the potential for employee exposure to hazardous chemicals.
"Reproductive toxins" means chemicals which affect the reproductive capabilities including chromosomal damage (mutations)and effects on fetuses (teratogenesis).
"Select carcinogen" means any substance which meets one of the following criteria:
"Water-reactive" means a chemical that reacts with water to release a gas that is either flammable or presents a health hazard.
For laboratory uses of OSHA regulated substances, the employer shall assure that laboratory employees' exposures to such substances do not exceed the permissible exposure limits specified in 29 CFR part 1910, subpart Z.
General. (Appendix A of this section is non-mandatory but provides guidance to assist employers in the development of the Chemical Hygiene Plan).
(i) Capable of protecting employees from health hazards associated with hazardous chemicals in that laboratory and
(ii) Capable of keeping exposures below the limits specified n paragraph (c) of this section.
(i) Standard operating procedures relevant to safety and health considerations to be followed when laboratory work involves the use of hazardous chemicals;
(ii) Criteria that the employer will use to determine and implement control measures to reduce employee exposure to hazardous chemicals including engineering controls, the use of personal protective equipment and hygiene practices; particular attention shall be given to the selection of control measures for chemicals that are known to be extremely hazardous;
(iii) A requirement that fume hoods and other protective equipment are functioning properly and specific measures that shall be taken to ensure proper and adequate performance of such equipment;
(iv) Provisions for employee information and training as prescribed in paragraph (f) of this section;
(v) The circumstances under which a particular laboratory operation, procedure or activity shall require prior approval from the employer or the employer's designee before implementation;
(vi) Provisions for medical consultation and medical examinations in accordance with paragraph (g) of this section;
(vii) Designation of personnel responsible for implementation of the Chemical Hygiene Plan including the assignment of a Chemical Hygiene Officer, and, if appropriate, establishment of a Chemical Hygiene Committee; and
(viii) Provisions for additional employee protection for work with particularly hazardous substances. These include "select carcinogens," reproductive toxins and substances which have a high degree of acute toxicity. Specific consideration shall be given to the following provisions which shall be included where appropriate:
(i) The contents of this standard and its appendices which shall be made available to employees;
(ii) the location and availability of the employer's Chemical Hygiene Plan;
(iii) The permissible exposure limits for OSHA regulated substances or recommended exposure limits for other hazardous chemicals where there is no applicable OSHA standard;
(iv) Signs and symptoms associated with exposures to hazardous chemicals used in the laboratory; and
(v) The location and availability of known reference material on the hazards, safe handling, storage and disposal of hazardous chemicals found in the laboratory including, but not limited to, Material Safety Data Sheets received from the chemical supplier.
(i) Employee training shall include:
(A) Methods and observations that may be used to detect the presence or release of a hazardous chemical (such as monitoring conducted by the employer, continuous monitoring devices, visual appearance or odor of hazardous chemicals when being released, etc.);
(B) The physical and health hazards of chemicals in the work area; and
(C) The measures employees can take to protect themselves from these hazards, including specific procedures the employer has implemented to protect employees from exposure to hazardous chemicals, such as appropriate work practices, emergency procedures, and personal protective equipment to be used.
(ii) The employee shall be trained on the applicable details of the employer's written Chemical Hygiene Plan.
(i) Whenever an employee develops signs or symptoms associated with a hazardous chemical to which the employee may have been exposed in the laboratory, the employee shall be provided an opportunity to receive an appropriate medical examination.
(ii) Where exposure monitoring reveals an exposure level routinely above the action level (or in the absence of an action level, the PEL) for an OSHA regulated substance for which there are exposure monitoring and medical surveillance requirements, medical surveillance shall be established for the affected employee as prescribed by the particular standard.
(iii) Whenever an event takes place in the work area such as a spill, leak, explosion or other occurrence resulting in the likelihood of a hazardous exposure, the affected employee shall be provided an opportunity for a medical consultation. Such consultation shall be for the purpose of determining the need for a medical examination.
(i) The identity of the hazardous chemical(s) to which the employee may have been exposed;
(ii) A description of the conditions under which the exposure occurred including quantitative exposure data, if available; and
(iii) A description of the signs and symptoms of exposure that the employee is experiencing, if any.
(i) For examination or consultation required under this standard, the employer shall obtain a written opinion from the examining physician which shall include the following:
(A) Any recommendation for further medical follow-up;
(B) The results of the medical examination and any associated tests;
(C) Any medical condition which may be revealed in the course of the examination which may place the employee at increased risk as a result of exposure to a hazardous workplace; and
(D) A statement that the employee has been informed by the physician of the results of the consultation or medical examination and any medical condition that may require further examination or treatment
(ii) The written opinion shall not reveal specific findings of diagnoses unrelated to occupational exposure.
(i) Employers shall ensure that labels on incoming containers of hazardous chemicals are not removed or defaced.
(ii) Employers shall maintain any material safety data sheets that are received with incoming shipments of hazardous chemicals, and ensure that they are readily accessible to laboratory employees.
(i) If the composition of the chemical substance which is produced exclusively for the laboratory's use is known, the employer shall determine if it is a hazardous chemical as defined in paragraph(b) of this section. If the chemical is determined to be hazardous, the employer shall provide appropriate training as required under paragraph (f) of this section.
(ii) If the chemical produced is a byproduct whose composition is not known, the employer shall assume that the substance is hazardous and shall implement paragraph (e) of this section.
(iii) If the chemical substance is produced for another user outside of the laboratory, the employer shall comply with the Hazard Communication Standard (29 CFR 1910.120) including the requirements for preparation of material safety data sheets and labeling.
Where the use of respirators is necessary to maintain exposure below permissible exposure limits, the employer shall provide, at no cost to the employee, the proper respiratory equipment. Respirators shall be selected and used in accordance with the requirements of 29 CFR 1910.134.
(i) Employers shall have developed and implemented a written Chemical Hygiene Plan no later than January 31, 1991
ii) Paragraph (a)(2) of this section shall not take effect until the employer has developed and implemented a written Chemical Hygiene Plan.
The information contained in the appendices is not intended, by itself, to create any additional obligations not otherwise imposed or to detract from any existing obligation.
Table of Contents
Corresponding Sections of the Standard and This Appendix
A. General Principles
C. The Laboratory Facility
D. Components of the Chemical Hygiene Plan
E. General Procedures for Working With Chemicals
F. Safety Recommendations
G. Material Safety Data Sheets
As guidance for each employer's development of an appropriate laboratory Chemical Hygiene Plan, the following non-mandatory recommendations are provided. They were extracted from "Prudent Practices" for Handling Hazardous Chemicals in Laboratories"(referred to below as "Prudent Practices"), which was published in 1981 by the National Research Council and is available from the National Academy Press, 2101 Constitution Ave., NW,.Washington DC 20418.
"Prudent Practices" is cited because of its wide distribution and acceptance and because of its preparation by members of the laboratory community through the sponsorship of the National Research Council. However, none of the recommendations given here will modify any requirements of the laboratory standard. This Appendix merely presents pertinent recommendations from "Prudent Practices", organized into a form convenient for quick reference during operation of a laboratory facility and during development and application of a Chemical Hygiene Plan. Users of this appendix should consult "Prudent Practices" for a more extended presentation and justification for each recommendation.
"Prudent Practices" deal with both safety and chemical hazards while the laboratory standard is concerned primarily with chemical hazards. Therefore, only those recommendations directed primarily toward control of toxic exposures are cited in this appendix, with the term "chemical hygiene" being substituted for the word "safety". However, since conditions producing or threatening physical injury often pose toxic risks as well, page references concerning major categories of safety hazards in the laboratory are given in section F.
The recommendations from "Prudent Practices" have been paraphrased, combined, or otherwise reorganized, and headings have been added. However, their sense has not been changed.
Corresponding Sections of the Standard and this Appendix
The following table is given for the convenience of those who are developing a Chemical Hygiene Plan which will satisfy the requirements of paragraph (e) of the standard. It indicates those sections of this appendix which are most pertinent to each of the sections of paragraph (e) and related paragraphs.
Topic in laboratory standard
Relevant appendix section
|(e)(3)(i)||Standard operating procedures for handling toxic chemicals.||C, D, E|
|(e)(3)(ii)||Criteria to be used for implementation of measures to reduce exposures.||D|
|(e)(3)(iii)||Fume hood performance||C4b|
|(e)(3)(iv)||Employee information and training (including emergency procedures).||D10, D9|
|(e)(3)(v)||Requirements for prior approval of laboratory activities.||E2b, E4b|
|(e)(3)(vi)||Medical consultation and medical examinations.||D5, E4f|
|(e)(3)(vii)||Chemical hygiene responsibilities.||B|
|(e)(3)(viii)||Special precautions for work with particularly hazardous substances.||E2, E3, E4|
In this appendix, those recommendations directed primarily at administrators and supervisors are given in sections A - D. Those recommendations of primary concern to employees who are actually handling laboratory chemicals are given in section E. (Reference to page numbers in "Prudent Practices" are given in parentheses.)
In addition to the more detailed recommendations listed below in sections B-E, "Prudent Practices" expresses certain general principles, including the following:
Responsibility for chemical hygiene rests at all levels (6,11, 21) including the:
(a) Work with administrators and other employees to develop and
(b) Monitor procurement, use, and disposal of chemicals used in the lab (8);
(c) See that appropriate audits are maintained (8);
(d) Help project directors develop precautions and adequate facilities(10);
(e) Know the current legal requirements concerning regulated substances (50); and
(f) Seek ways to improve the chemical hygiene program (8, 11).
(a) Ensure that workers know and follow the chemical hygiene rules, that protective equipment is available and in working order, and that appropriate training has been provided (21, 22);
(b) Provide regular, formal chemical hygiene and housekeeping inspections including routine inspections of emergency equipment(21, 171);
(c) Know the current legal requirements concerning regulated substances (50, 231);
(d) Determine the required levels of protective apparel and equipment(156, 160, 162); and
(e) Ensure that facilities and training for use of any material being ordered are adequate (215).
(a) Planning and conducting each operation in accordance with the institutional chemical hygiene procedures (7, 21, 22, 230);and
(b) Developing good personal chemical hygiene habits (22).
(a) An appropriate general ventilation system (see C4 below)with air intakes and exhausts located so as to avoid intake of contaminated air (194);
(b) Adequate, well-ventilated stockrooms/storerooms (218, 219);
(c) Laboratory hoods and sinks (12, 162);
(d) Other safety equipment including eyewash fountains and drench showers (162, 169); and
(e) Arrangements for waste disposal (12, 240).
(a) General laboratory ventilation. This system should: Provide a source of air for breathing and for input to local ventilation devices (199); it should not be relied on for protection from toxic substances released into the laboratory(198); ensure that laboratory air is continually replaced, preventing increase of air concentrations of toxic substances during the working day (194); direct air flow into the laboratory from non-laboratory areas and out to the exterior of the building (194).
(b) Hoods. A laboratory hood with 2.5 linear feet of hood space per person should be provided for every 2 workers if they spend most of their time working with chemicals (199); each hood should have a continuous monitoring device to allow convenient confirmation of adequate hood performance before use (200, 209). If this is not possible, work with substances of unknown toxicity should be avoided (13) or other types of local ventilation devices should be provided (199). See pp. 201-206 for a discussion of hood design, construction, and evaluation.
(c) Other local ventilation devices. Ventilated storage cabinets, canopy hoods, snorkels, etc. should be provided as needed (199). Each canopy hood and snorkel should have a separate exhaust duct(207).
(d) Special ventilation areas. Exhaust air from glove boxes and isolation rooms should be passed through scrubbers or other treatment before release into the regular exhaust system (208). Cold rooms and warm rooms should have provisions for rapid escape and for escape in the event of electrical failure (209).
(e) Modifications. Any alteration of the ventilation system should be made only if thorough testing indicates that worker protection from airborne toxic substances will continue to be adequate (12, 193, 204).
(f) Performance. Rate: 4-12 room air changes/hour is normallyadequate general ventilation if local exhaust systems such as hoods are used as the primary method of control (194).
(g) Quality. General air flow should not be turbulent and should be relatively uniform throughout the laboratory, with no high velocity or static areas (194, 195); airflow into and within the hood should not be excessively turbulent (200); hood face velocity should be adequate (typically 60-100 lfm) (200, 204).
(h) Evaluation. Quality and quantity of ventilation should be evaluated on installation (202), regularly monitored (at least every 3 months) (6, 12, 14, 195), and reevaluated whenever a change in local ventilation devices is made (12, 195, 207). See pp 195-198for methods of evaluation and for calculation of estimated airborne contaminant concentrations.
(a) Procurement. Before a substance is received, information on proper handling, storage, and disposal should be known to those who will be involved (215, 216). No container should be accepted without an adequate identifying label (216). Preferably, all substances should be received in a central location (216).
(b) Stockrooms/storerooms. Toxic substances should be segregated in a well-identified area with local exhaust ventilation (221). Chemicals which are highly toxic (227) or other chemicals whose containers have been opened should be in unbreakable secondary containers (219). Stored chemicals should be examined periodically(at least annually) for replacement, deterioration, and container integrity (218-19). Stockrooms/storerooms should not be used as preparation or repackaging areas, should be open during normal working hours, and should be controlled by one person (219).
(c) Distribution. When chemicals are hand carried, the container should be placed in an outside container or bucket. Freight-only elevators should be used if possible (223).
(d) Laboratory storage. Amounts permitted should be as small as practical. Storage on bench tops and in hoods is inadvisable. Exposure to heat or direct sunlight should be avoided. Periodic inventories should be conducted, with unneeded items being discarded or returned to the storeroom/stockroom (225-6, 229).
Regular instrumental monitoring of airborne concentrations is not usually justified or practical in laboratories but may be appropriate when testing or redesigning hoods or other ventilation devices (12) or when a highly toxic substance is stored or used regularly (e.g., 3 times/week) (13).
(a) Cleaning. Floors should be cleaned regularly (24).
(b) Inspections. Formal housekeeping and chemical hygiene inspections should be held at least quarterly (6, 21) for units which have frequent personnel changes and semiannually for others; informal inspections should be continual (21).
(c) Maintenance. Eye wash fountains should be inspected at intervals of not less than 3 months (6). Respirators for routine use should be inspected periodically by the laboratory supervisor (169). Other safety equipment should be inspected regularly. (e.g., every 3-6 months) (6, 24, 171). Procedures to prevent restarting of out-of-service equipment should be established (25).
(d) Passageways. Stairways and hallways should not be used as storage areas (24). Access to exits, emergency equipment, and utility controls should never be blocked (24).
(a) Compliance with regulations. Regular medical surveillance should be established to the extent required by regulations (12).
(b) Routine surveillance. Anyone whose work involves regular and frequent handling of toxicologically significant quantities of a chemical should consult a qualified physician to determine on an individual basis whether a regular schedule of medical surveillance is desirable (11, 50).
c) First aid. Personnel trained in first aid should be available during working hours and an emergency room with medical personnel should be nearby (173). See pp. 176-178 for description of some emergency first aid procedures.
These should include for each laboratory:
(a) Protective apparel compatible with the required degree of protection for substances being handled (158-161);
(b) An easily accessible drench-type safety shower (162, 169);
(c) An eyewash fountain (162)
(d) A fire extinguisher (162-164);
(e) Respiratory protection (164-9), fire alarm and telephone for emergency use (162) should be available nearby; and
(f) Other items designated by the laboratory supervisor (156,160).
(a) Accident records should be written and retained (174).
(b) Chemical Hygiene Plan records should document that the facilities and precautions were compatible with current knowledge and regulations(7).
(c) Inventory and usage records for high-risk substances should be kept as specified in sections E3e below.
(d) Medical records should be retained by the institution in accordance with the requirements of state and federal regulations(12).
Prominent signs and labels of the following types should be posted:
(a) Emergency telephone numbers of emergency personnel/facilities, supervisors, and laboratory workers (28);
(b) Identity labels, showing contents of containers (including waste receptacles) and associated hazards (27, 48);
(c) Location signs for safety showers, eyewash stations, other safety and first aid equipment, exits (27) and areas where food and beverage consumption and storage are permitted (24); and
(d) Warnings at areas or equipment where special or unusual hazards exist (27).
(a) A written emergency plan should be established and communicated to all personnel; it should include procedures for ventilation failure (200), evacuation, medical care, reporting, and drills(172).
(b) There should be an alarm system to alert people in all parts of the facility including isolation areas such as cold rooms (172).
(c) A spill control policy should be developed and should include consideration of prevention, containment, cleanup, and reporting(175).
(d) All accidents or near accidents should be carefully analyzed with the results distributed to all who might benefit (8, 28).
(a) Aim: To assure that all individuals at risk are adequately informed about the work in the laboratory, its risks, and what to do if an accident occurs (5, 15).
(b) Emergency and Personal Protection Training: Every laboratory worker should know the location and proper use of available protective apparel and equipment (154, 169). Some of the full-time personnel of the laboratory should be trained in the proper use of emergency equipment and procedures (6). Such training as well as first aid instruction should be available to (154) and encouraged for(176) everyone who might need it.
(c) Receiving and stockroom/storeroom personnel should know about hazards, handling equipment, protective apparel, and relevant regulations (217).
(d) Frequency of Training: The training and education programs should be a regular, continuing activity - not simply an annual presentation (15).
(e) Literature/Consultation: Literature and consulting advice concerning chemical hygiene should be readily available to laboratory personnel, who should be encouraged to use these information resources(14).
(a) Aim: To assure that minimal harm to people, other organisms, and the environment will result from the disposal of waste laboratory chemicals (5)
(b) Content (14, 232, 233, 240): The waste disposal program should specify how waste is to be collected, segregated, stored, and transported and include consideration of what materials can be incinerated. Transport from the institution must be in accordance with DOT regulations (244).
(c) Discarding Chemical Stocks: Unlabeled containers of chemicals and solutions should undergo prompt disposal; if partially used, they should not be opened (24, 27). Before a worker's employment in the laboratory ends, chemicals for which that person was responsible should be discarded or returned to storage (226).
(d) Frequency of Disposal: Waste should be removed from laboratories to a central waste storage area at least once per week and from the central waste storage area at regular intervals (14).
(e) Method of Disposal: Incineration in an environmentally acceptable manner is the most practical disposal method for combustible laboratory waste (14, 238, 241). Indiscriminate disposal by pouring waste chemicals down the drain (14, 231, 242) or adding them to mixed refuse for landfill burial is unacceptable (14). Hoods should not be used as a means of disposal for volatile chemicals(40,200). Disposal by recycling (233, 243) or chemical decontamination(40, 230) should be used when possible.
The Chemical Hygiene Plan should require that laboratory workers know and follow its rules and procedures. In addition to the procedures of the sub programs mentioned above, these should include the rules listed below.
The following should be used for essentially all laboratory work with chemicals:
(a) Accidents and spills - Eye Contact: Promptly flush eyes with water for a prolonged period (15 minutes) and seek medical attention(33, 172).
Ingestion: Encourage the victim to drink large amounts of water(178).
Skin Contact: Promptly flush the affected area with water (33,172, 178) and remove any contaminated clothing (172, 178). If symptoms persist after washing, seek medical attention (33). Clean-up. Promptly clean up spills, using appropriate protective apparel and equipment and proper disposal (24, 33). See pp. 233-237for specific clean-up recommendations.
(b) Avoidance of "routine" exposure: Develop and encourage safe habits (23); avoid unnecessary exposure to chemicals by any route (23). Do not smell or taste chemicals (32). Vent apparatus which may discharge toxic chemicals (vacuum pumps, distillation columns, etc.) into local exhaust devices (199). Inspect gloves(157) and test glove boxes (208) before use. Do not allow release of toxic substances in cold rooms and warm rooms, since these have contained recirculated atmospheres (209).
(c) Choice of chemicals: Use only those chemicals for which the quality of the available ventilation system is appropriate (13).
(d) Eating, smoking, etc.: Avoid eating, drinking, smoking, gum chewing, or application of cosmetics in areas where laboratory chemicals are present (22, 24, 32, 40); wash hands before conducting these activities (23, 24). Avoid storage, handling, or consumption of food or beverages in storage areas, refrigerators, glassware or utensils which are also used for laboratory operations (23, 24, 226).
(e) Equipment and glassware: Handle and store laboratory glassware with care to avoid damage; do not use damaged glassware (25). Use extra care with Dewar flasks and other evacuated glass apparatus; shield or wrap them to contain chemicals and fragments should implosion occur (25). Use equipment only for its designed purpose(23, 26).
(f) Exiting: Wash areas of exposed skin well before leaving the laboratory (23).
(g) Horseplay: Avoid practical jokes or other behavior which might confuse, startle or distract another worker (23).
(h) Mouth suction: Do not use mouth suction for pipeting or starting a siphon (23, 32).
(i) Personal apparel: Confine long hair and loose clothing (23,158). Wear shoes at all times in the laboratory but do not wear sandals, perforated shoes, or sneakers (158).
(j) Personal housekeeping: Keep the work area clean and uncluttered, with chemicals and equipment being properly labeled and stored; clean up the work area on completion of an operation or at the end of each day (24).
(k) Personal protection: Assure that appropriate eye protection(154-156) is worn by all persons, including visitors, where chemicals are stored or handled (22, 23, 33, 154). Wear appropriate gloves when the potential for contact with toxic materials exists (157);inspect the gloves before each use, wash them before removal, and replace them periodically (157). (A table of resistance to chemicals of common glove materials is given p. 159). Use appropriate(164-168) respiratory equipment when air contaminant concentrations are not sufficiently restricted by engineering controls (164-5),inspecting the respirator before use (169). Use any other protective and emergency apparel and equipment as appropriate (22, 157-162). Avoid use of contact lenses in the laboratory unless necessary; if they are used, inform supervisor so special precautions can be taken (155). Remove laboratory coats immediately on significant contamination (161).
(l) Planning: Seek information and advice about hazards (7),plan appropriate protective procedures, and plan positioning of equipment before beginning any new operation (22, 23).
(m) Unattended operations: Leave lights on, place an appropriate sign on the door, and provide for containment of toxic substances in the event of failure of a utility service (such as cooling water) to an unattended operation (27, 128).
(n) Use of hood: Use the hood for operations which might resulting release of toxic chemical vapors or dust (198-9). As a rule of thumb, use a hood or other local ventilation device when working with any appreciably volatile substance with a TLV of less than 50 ppm (13). Confirm adequate hood performance before use; keep hood closed at all times except when adjustments within the hood are being made (200); keep materials stored in hoods to a minimum and do not allow them to block vents or air flow(200). Leave the hood "on" when it is not in active use if toxic substances are stored in it or if it is uncertain whether adequate general laboratory ventilation will be maintained when it is "off" (200).
(o) Vigilance: Be alert to unsafe conditions and see that they are corrected when detected (22).
(p) Waste disposal: Assure that the plan for each laboratory operation includes plans and training for waste disposal (230). Deposit chemical waste in appropriately labeled receptacles and follow all other waste disposal procedures of the Chemical Hygiene Plan (22, 24). Do not discharge to the sewer concentrated acids or bases (231); highly toxic, malodorous, or lachrymatory substances(231); or any substances which might interfere with the biological activity of waste water treatment plants, create fire or explosion hazards, cause structural damage or obstruct flow (242).
(q) Working alone: Avoid working alone in a building; do not work alone in a laboratory if the procedures being conducted are hazardous (28).
(a) Allergens (examples: diazomethane, isocyanates, bichromates):Wear suitable gloves to prevent hand contact with allergens orsubstances of unknown allergenic activity (35).
(b) Embryotoxins (34-5) (examples: organomercurials, lead compounds,formamide): If you are a woman of childbearing age, handle these substances only in a hood whose satisfactory performance has been confirmed, using appropriate protective apparel (especially gloves)to prevent skin contact. Review each use of these materials with the research supervisor and review continuing uses annually or whenever a procedural change is made. Store these substances, properly labeled, in an adequately ventilated area in an unbreakable secondary container. Notify supervisors of all incidents of exposure or spills; consult a qualified physician when appropriate.
Examples: diisopropylfluorophosphate (41), hydrofluoric acid(43),hydrogen cyanide (45).
Supplemental rules to be followed in addition to those mentioned above (Procedure B of "Prudent Practices", pp. 39-41):
(a) Aim: To minimize exposure to these toxic substances by any route using all reasonable precautions (39).
(b) Applicability: These precautions are appropriate for substances with moderate chronic or high acute toxicity used in significant quantities (39).
(c) Location: Use and store these substances only in areas of restricted access with special warning signs (40, 229). Always use a hood (previously evaluated to confirm adequate performance with a face velocity of at least 60 linear feet per minute) (40)or other containment device for procedures which may result in the generation of aerosols or vapors containing the substance(39); trap released vapors to prevent their discharge with the hood exhaust (40).
(d) Personal protection: Always avoid skin contact by use of gloves and long sleeves (and other protective apparel as appropriate)(39). Always wash hands and arms immediately after working with these materials (40).
(e) Records: Maintain records of the amounts of these materials on hand, amounts used, and the names of the workers involved (40,229).
(f) Prevention of spills and accidents: Be prepared for accidents and spills (41). Assure that at least 2 people are present at all times if a compound in use is highly toxic or of unknown toxicity(39). Store breakable containers of these substances in chemically resistant trays; also work and mount apparatus above such trays or cover work and storage surfaces with removable, absorbent, plastic backed paper (40). If a major spill occurs outside the hood, evacuate the area; assure that cleanup personnel wear suitable protective apparel and equipment (41).
(g) Waste: Thoroughly decontaminate or incinerate contaminated clothing or shoes (41). If possible, chemically decontaminate by chemical conversion (40). Store contaminated waste in closed, suitably labeled, impervious containers (for liquids, in glass or plastic bottles half-filled with vermiculite) (40).
(Examples: dimethylmercury and nickel carbonyl (48), benzo-a-pyrene
(51), N-nitrosodiethylamine (54), other human carcinogens or substances
with high carcinogenic potency in animals (38).)
Further supplemental rules to be followed, in addition to all these mentioned above, for work with substances of known high chronic toxicity (in quantities above a few milligrams to a few grams, depending on the substance) (47). (Procedure A of "PrudentPractices" pp. 47-50).
(a) Access: Conduct all transfers and work with these substances in a "controlled area": a restricted access hood, glovebox, or portion of a lab, designated for use of highly toxic substances, for which all people with access are aware of the substances being used and necessary precautions (48).
(b) Approvals: Prepare a plan for use and disposal of these materials and obtain the approval of the laboratory supervisor (48).
(c) Non-contamination/Decontamination: Protect vacuum pumps against contamination by scrubbers or HEPA filters and vent them into the hood (49). Decontaminate vacuum pumps or other contaminated equipment, including glassware, in the hood before removing them from the controlled area (49, 50). Decontaminate the controlled area before normal work is resumed there (50).
(d) Exiting: On leaving a controlled area, remove any protective apparel (placing it in an appropriate, labeled container) and thoroughly wash hands, forearms, face, and neck (49).
(e) Housekeeping: Use a wet mop or a vacuum cleaner equipped with a HEPA filter instead of dry sweeping if the toxic substance was a dry powder (50).
(f) Medical surveillance: If using toxicologically significant quantities of such a substance on a regular basis (e.g., 3 times per week), consult a qualified physician concerning desirability of regular medical surveillance (50).
(g) Records: Keep accurate records of the amounts of these substances stored (229) and used, the dates of use, and names of users (48).
(h) Signs and labels: Assure that the controlled area is conspicuously marked with warning and restricted access signs (49) and that all containers of these substances are appropriately labeled with identity and warning labels (48).
(i) Spills: Assure that contingency plans, equipment, and materials to minimize exposures of people and property in case of accident are available (233-4).
(j) Storage: Store containers of these chemicals only in a ventilated limited access (48, 227, 229) area in appropriately labeled, unbreakable, chemically resistant, secondary containers (48, 229).
(k) Glove boxes: For a negative pressure glove box, ventilation rate must be at least 2 volume changes/hour and pressure at least0.5 inches of water (48). For a positive pressure glove box, thoroughly check for leaks before each use (49). In either case, trap the exit gases or filter them through a HEPA filter and then release them into the hood (49).
(l) Waste: Use chemical decontamination whenever possible; ensure that containers of contaminated waste (including washings from contaminated flasks) are transferred from the controlled area in a secondary container under the supervision of authorized personnel(49, 50, 233).
(a) Access: For large scale studies, special facilities with restricted access are preferable (56)
(b) Administration of the toxic substance: When possible, administer the substance by injection or gavage instead of in the diet. If administration is in the diet, use a caging system under negative pressure or under laminar air flow directed toward HEPA filters(56).
(c) Aerosol suppression: Devise procedures which minimize formation and dispersal of contaminated aerosols, including those from food, urine, and feces (e.g., use HEPA filtered vacuum equipment for cleaning, moisten contaminated bedding before removal from the cage, mix diets in closed containers in a hood) (55, 56).
(d) Personal protection: When working in the animal room, wear plastic or rubber gloves, fully buttoned laboratory coat or jumpsuit and, if needed because of incomplete suppression of aerosols, other apparel and equipment (shoe and head coverings, respirator)(56).
(e) Waste disposal: Dispose of contaminated animal tissues and excreta by incineration if the available incinerator can convert the contaminant to non-toxic products (238); otherwise, package the waste appropriately for burial in an EPA-approved site (239).
The above recommendations from "Prudent Practices" do not include those which are directed primarily toward prevention of physical injury rather than toxic exposure. However, failure of precautions against injury will often have the secondary effect of causing toxic exposures. Therefore, we list below page references for recommendations concerning some of the
major categories of safety hazards which also have implications for chemical hygiene:
Material safety data sheets are presented in "Prudent Practices" for the chemicals listed below. (Asterisks denote that comprehensive material safety data sheets are provided).
o Acetyl peroxide (105)
o Acrolein (106)
o Aniline (109)
o Benzene (110)
o Benzo[a]pyrene (112)
o Bis(chloromethyl) ether (113)
Boron trichloride (91)
Boron trifluoride (92)
o Tert-butyl hydroperoxide (148)
o Carbon disulfide (116)
Carbon monoxide (92)
o Carbon tetrachloride (118)
Chlorine trifluoride (94)
o Chloroform (121)
o Diethyl ether (122)
Diisopropyl fluorophosphate (41)
o Dimethylformamide (123)
o Dimethyl sulfate (125)
o Dioxane (126)
o Ethylene dibromide (128)
o Fluorine (95)
o Formaldehyde (130)
o Hydrazine and salts (132)
Hydrofluoric acid (43)
Hydrogen bromide (98)
Hydrogen chloride (98)
o Hydrogen cyanide (133)
o Hydrogen sulfide (135)
Mercury and compounds (52)
o Methanol (137)
o Morpholine (138)
o Nickel carbonyl (99)
o Nitrobenzene (139)
Nitrogen dioxide (100)
o Peracetic acid (141)
o Phenol (142)
o Phosgene (143)
o Pyridine (144)
o Sodium azide (145)
o Sodium cyanide (147)
Sulfur dioxide (101)
o Trichloroethylene (149)
o Vinyl chloride (150)
29 CFR 1910.1450 App B References (Non-Mandatory)
The following references are provided to assist the employer in the development of a Chemical Hygiene Plan. The materials listed below are offered as non-mandatory guidance. References listed here do not imply specific endorsement of a book, opinion, technique, policy or a specific solution for a safety or health problem. Other references not listed here may better meet the needs of a specific laboratory.
(a) Materials for the development of the Chemical Hygiene Plan:
(b) Hazardous Substances Information:
(c) Information on Ventilation:
(d) Information on Availability of Referenced Material:
(Approved by the Office of Management and Budget under control number 1218-0131)
[55 FR 3327, Jan. 31, 1990]