LCQ/ Advantage HPLC-Mass Spectrotometer

Operating Manual

Table of Contents

(The TOC is a set of links into .pdf files that are bookmarked according to the items listed in the TOC)

LCQ/Advantage

Table of Contents

Introduction   

         1-1    Why Use the LCQ Advantage MS Detector?

         1-2    Which MS Detector Technique-ESI or APCI-Is Better for Analyzing My Samples?

                        Using ESI/MS

                        Using APCI/MS

         1.3    Should I Use Auxiliary Gas or Sweep Gas?

         1.4    How Can I Introduce My Samples into the MS Detector?

         1.5    What Types of Buffers Should I Use? What Types Should I Avoid?

         1.6    How Should I Set Up the MS Detector for Various LC Flow Rates?

         1.7    What Is Tuning and Calibration of the MS Detector All About?

         1.8    What Types of Experiments Can I Perform with LCQ Advantage?

                        General MS or MS/MS Experiments

                        Data-Dependent Experiments

Setting Up for Tuning and Calibrating the MS Detector in ESI/MS Mode

         2.1    Removing the APCI Probe Assembly

         2.2    Connecting the PEEK Safety Sleeve and a New Fused-Silica Sample Tube to the

                        ESI Probe

         2.3    Installing the ESI Probe Assembly

         2.4    Setting Up the Syringe Pump for Tuning and Calibrating

         2.5    Setting Up the MS Detector for Tuning and Calibrating  

Tuning with Your Analyte in LC/ESI/MS Mode 

         4.1    Setting Up to Introduce Sample by Syringe Pump into Solvent Flow from an LC

         4.2    Setting Up to Tune the MS Detector with Your Analyte

         4.3    Optimizing the MS Detector Tune Automatically with Your Analyte

         4.4    Saving the ESI/MS Tune Method13

Acquiring ESI Sample Data Using the Tune Plus Window 

         5.1    Setting Up to Introduce Sample by Loop Injection into Solvent Flow from an LC

         5.2    Acquiring MS Data in the SIM Scan Type

         5.3    Setting Up to Acquire MS/MS Data in the Full Scan Type

                        Setting Up to Optimize Collision Energy

                        Optimizing the Collision Energy Automatically for an MS/MS Experiment

Setting Up to Acquire Data in the APCI/MS Mode

         6.1    Removing the ESI Probe Assembly2

         6.2    Installing the APCI Probe Assembly

         6.3    Setting Up the Inlet for Tuning Using High-Flow Infusion

         6.4    Setting Up the MS Detector for APCI/MS Operation8

Optimizing the MS Detector with Your Analyte in APCI/MS Mode

         7.1    Optimizing the Tune of the MS Detector Automatically in APCI/MS Mode

         7.2    Saving the APCI/MS Tune Method

         7.3    Cleaning the MS Detector after Tuning in APCI Mode

Acquiring APCI Sample Data Using the Tune Plus Window

         8.1    Setting Up to Introduce Sample by Loop Injection into Solvent flow from an LC

         8.2    Acquiring APCI Data in the SIM Scan Mode

                 LCO Advantage Getting Started