Abbreviated Operations Manual OLIS DM 45 Spectrofluorimeter

Abbreviated Operations Manual OLIS DM 45 Spectrofluorimeter     

The OLIS DM 45 is modern fluorimeter with the ability to scan both the excitation and emission monochromators.  Its basic setup is illustrated below:  

How Do I ??? Questions:

Read the Operations manual first, then go to the following link:  How Do I ????

Operations:  

Power up the DM45 by first turning on the Excitation Source power supply (LPS-220B), by pressing the red power button on the front of the power supply.  After a brief delay, a green light on the top of the Excitation Source should light and the power supply should register about 5.5 amps. 

Turn on the computer system by pressing the power button on the module below the monitor.  The computer will boot up into MS-DOS.  At the C:\> prompt, type olis-pcf.  

The control software is a DOS based, menu driven software. You should get a screen with the following choices:

S – Scan Data Collection Module

F – Scan Data Fit Module P – Assay Polarization Module   

N – Data Points per second    (default  N = 200)

D – Data Directory     (default   C:\TESTDATA )

C – Printer                    (default HP LaserJet)

By selecting ‘S’ you will move to the Scan Data Collection Module

Selecting ‘F’ you will move to the Scan Data Fit Module

Selecting ‘D’ allows you to change the directory your data will be stored in

Scan Data Collection

Module Command Module 

V – Data Collection                                 E – Erase screen, Display Data |
P – Set DVM Parameters                        H – Hash mark/Grid setup
                                                               F – Full Screen to Printer  

‘F6’ – Data Fit Module                          S – Storage/Retrieval
‘F5’ – Start-up Module                          D – Data DIR=C:\TESTDATA
Z – Terminate                                        O – MS DOS Command
                                                              L – List Disk File Directory  

‘F6’ is a toggle which takes you to the Data Fitting Module.

“V” takes you to the Data Collection Module.  The others are quite self explanatory.

‘S’ from the Command Module, takes you to the Retrieve/Store area which allows you to retrieve already stored data, or save data you have collected.  

Each of these commands then take you to another module as listed below.  Only the ones required to collect simple fluorescence data are included.  

Data Collection: “V”  

Data Collection Module   

R = # Scans/Spectrum              1                       ‘Esc’ – Exit to Command Module
S = # Samples/Data Point         1                      W – Monitor Info
T – Timed Scans                                                 C – Collect Data
I – Integration Time                 0.1                      Y – Set Y axis                  
                                                                         D – Collect Dark Current                       

In this module you are able to set the number of scans that are averaged for the final spectra ‘R’, set the number of times the emission is measured for each data point ‘S’, and set the integration time for each measurement ‘I’.   In most cases, the default values will work well.   

In order to collect the data, you need to set up the monochromators.  You can scan the excitation monochromator or the emission monochromator, but not both.  To set up the monochromator,  from the Data Collection Window press ‘W’; the following window will open:     

Data Collection  

Data Collection Parameters                                V – Return to Menu
200 Emission Data Points                                  W – Wavelength Ramp (Emission)
Scanning from 600 to 400 nm                                        current 600 to 400
Holding Excitation @ 350 nm                             M – Move Monochromator
Ave of 1 Scan(s) Rds/Datam  1                         C – Calibrate Monochromator  

Selecting ‘W’ will allow you to set up the monochromators to scan as you desire.  The following will be asked on the screen.  Enter the higher wavelength first as the instrument always scans from higher to lower.

                 Emission Wavelength                                     Excitation Wavelength               

Upper (<1000) = (type in value)                        Upper (<1000) = (type in value)             
Lower (>0    ) = (type in value)                           Lower (>0   ) = (type in value)    

To hold the Wavelength constant, enter the same value for both the upper and lower values.  

When the values are intered, you will then return  to the Data Collection Module.  Pressing ‘C’, will initiate a scan.  If you have selected multiple scans, then all those scans will be run.   Once the scans have run, the following box will appear above the scan.

Data Collection Parameters  

G – Go to next scan                              C – Cursor                                V – DVM
B – Assign current as baseline               M – Memory                            D – Disk    

The most important actions from this page are ‘C’ which presents a cursor on the scan which can be moved by the < and > keys to determine the wavelength and emission.  The ‘V’ key takes you back to the Command Module.  The ‘D’ key takes you to the storage  module which allows you to store the spectra to disk.  

If you press ‘D’ you get the following box:    

Enter any legal MS-DOS filename                      Enter File Name
Or    
Press ‘ Enter’ to list files in current directory      
Press ‘Esc’ to escape  

From the Command Module, pressing F6  or from the opening screen pressing ‘F’ will take you to the Data Fitting Module.       

Data Fitting Module  

V – Create Data (VersaFit)                               E – Erase Screen, Display Data
R – Data Reduction Routines                             H – Hash mark/Grid setup
                                                                         M – Change Axis Label
                                                                        W – Wavelength/Wavenumber Toggle                                                                          F – Full Screen to Printer  

‘F6’ – Data Fit Module                                     S – Storage/Retrieval
‘F5’ – Start-up Module                                     D – Data DIR=C:\TESTDATA
Z – Terminate                                                    O – MS DOS Command
                                                                         L – List Disk File Directory    

From the Data Fitting Module if you select the ‘R’ function to reduce data.    

Data Reductions  

R – Other Manipulations Routines                     Single Operant Manipulation
‘Esc’ – Exit to Command Module                     L – Log            S – Smooth
.                                                                       I – Invert          M – Multicomponent .                                                                            D -  dA/dnM                Analysis   

Pressing ‘R’ toggles through two more boxes:  

Other Manipulations Routines  

R – Other Manipulations Routines                     * - Multiply                   +  - Add Constant
‘Esc’ – Exit to Command Module                     T - Transform .                                                                        .                                                                               

Other Manipulations Routines  

R – Other Manipulations Routines                     - - Subtract                    +  - Add
‘Esc’ – Exit to Command Module                     / - Divide                       * - Multiply  

Pressing ‘S’ takes you to a smooth screen.   

Smooth                           

Source ___________   Destination _________________                                                      

A – Smooth All
[ - Smooth Selected Limits     where Source and Destination represent Memory Slots (see Memory Slots below)  

Pressing ‘A’ gives you the chance to select the number of data points to use in the smoothing routine.  

Pressing ‘D’  at the Manipulations Routines allows you to take the 1-4th derivative of the current spectrum.  It too takes you to a screen asking for Source and Destination Memory Slots (see below).  You are then asked for the order of the derivative plot you desire.              

Memory Slots:  There are 32 memory slots that can hold results of data manipulations.   The original data is always stored in Slot 0.  For most of the data manipulations, you will then be asked for the Source slot (slot from which the data to be manipulated will come) and asked what Destination slot (the slot in which the results of the manipulation will be stored).  It is probably a good idea to write down what each slot contains (or add comments to the data) so that you will be able to go back to the data in that particular slot.  

Printing

Printing can be accomplished by holding down the Control key and pressing P; ^P.
The current screen will be printed to the printer.

How Do I ???   

How do I turn on the system? 
How do I set the excitation monochromator?
How do I set the emission monochromator?
How do I collect data?
How do I save the data collected?
How do I print the data collected?
How do I find the wavelength and emission values?
How do I smooth the data?